Cic edizioni internazionali
Muscle, Ligaments and Tendons Journal

Hyperglycemia induces osteogenic differentiation of bone marrow derived stem cells: an in vitro study

Original Article, 1 - 7
doi: 10.11138/mltj/2018.8.1.001
Tag this article
Abstract
Full text PDF
Introduction: The physiopathology of calcific tendinopathy (CT) is largely unknown. It could be the result of an active cell-mediated process.
Many endocrine and metabolic diseases may impair the homeostasis of the tendon. The present study investigated whether hyperglycemia may influence the differentiation of bone marrow
MSCs (bMSCs). The hypothesis is that high glucose levels may induce bone differentiation of MSCs.
Methods: Bone marrow (BM) was aspirated from the humeral head of three patients and concentrated. bMSCs were taken to the lab, counted, plated and grown to confluence. After 24 hrs cells were treated with MEM supplemented with low (5.0 mM), physiological (10 m M) and high (25 mM) glucose, (+) and (-) 10 m M insulin. Control cells were treated with MEM alone. Quantitative polymerase chain reaction (qPCR) was used to measure changes in gene expression levels specific for fibrocartilage in bMSCs.
Results: After 7 days, a significantly increased gene expression of collagen type I, type II, alkaline phosphatase and osteopontin was found in bMSCs supplemented with high glucose compared both to control group, and to low and physiological glucose groups with and without insulin (p<0.05). When insulin was added to high glucose culture, a significantly higher expression of aggrecan, alkaline phosphatase, type I and II collagen, and fibronectin was found compared to all the other groups (p<0.05).
Conclusion: When cultured in a high glucose medium, bMSCs express bone markers, and are able to differentiate toward an osteoblast lineage. CT may be caused by erroneous differentiation of MSCs in presence of high glucose serum levels.

Vol. 8 (No. 2) 2018 April/June

  1. Percutaneous trigger finger release with an MS64 scalpel
    Mifsut-Miedes D., Valverde Navarro A.A., Rivelles J.R.
    doi: 10.11138/mltj/2018.8.2.135
  2. The effect of choice reaction time task on pre-landing muscle timing in athletes with and without chronic ankle instability
    Fereydounnia S., Shadmehr A., Talebian Moghadam S., Olyaei G., Jalaie S.
    doi: 10.11138/mltj/2018.8.2.142
  3. Cigarette smoking on the functional recovery of patients with rotator cuff tear submitted to physical therapy
    Cardoso Leffa T., Galvão Novelli J., Biff dos Santos G., Maciel Bello G., da Silva Santos M., Martins Silveira M., Leindecker R.C., Boff Daitx R., Baptista Dohnert M.
    doi: 10.11138/mltj/2018.8.2.150
  4. The undefined anatomical variations of the deltoid ligament bundles: a cadaveric study
    Zamperetti M., Guelfi M., Biz C., Pantalone A., Salini V., Oliva X.M., Ruggieri P., Mirapeix R.M.
    doi: 10.11138/mltj/2018.8.2.163
  5. The reproducibility of manual and fixed tensioning in anterior cruciate ligament reconstruction
    Stevens R., Chandrasenan J., Ali F., Haslam P., Nicolaou N.
    doi: 10.11138/mltj/2018.8.2.172
  6. Arthroscopic management of anterior inferior iliac spine impingement: a systematic review
    Fioruzzi A., Jannelli E., Ivone A., Perelli S., Fontana A., Giai Via A.
    doi: 10.11138/mltj/2018.8.2.178
  7. Low revision rate and excellent subjective outcome of primary ACL repair with a minimum follow-up of 5 years
    Nau T., Teuschl A., Ebner A., Jung I., Schenk C.
    doi: 10.11138/mltj/2018.8.2.185
  8. Box-loop technique in the management of complex elbow instability: a prospective controlled trial
    Vicenti G., Solarino G., Carrozzo M., Bizzoca D., Marinelli A., Rotini R., Moretti B.
    doi: 10.11138/mltj/2018.8.2.191
  9. Simvastatin does not adversely affect Achilles tendon properties in a diet induced hypercholesterolemia rat model
    Choi D.S., Shetye S.S., Tucker J.J., Huegel J., Soslowsky L.J.
    doi: 10.11138/mltj/2018.8.2.199
  10. Quality assessment of muscle injury classification in sports: a systematic literature review
    SantAnna J.P.C., de Almeida A.M., Pedrinelli A., Hernandez A.J., Fernandes T.L.
    doi: 10.11138/mltj/2018.8.2.206
  11. Laser therapy penetration depth: a near-infrared study on a horse tendon model
    Monici M., Gnerucci A., Falconi T., Bani D., Cialdai F., Fusi F., Romano G.
    doi: 10.11138/mltj/2018.8.2.222
  12. Quantitative elastography of Achilles tendon using Shear Wave Elastography (SWE): correlation with zonal anatomy
    Petitpierre F., Perez J.T., Bise S., Fournier C., Hauger O., Dallaudière B.
    doi: 10.11138/mltj/2018.8.2.229
  13. Regional differences in anterior cruciate ligament imaging biomarkers: T2 and T2* values
    Schmitz R.J., Wang H-M., Kraft R.A., Shultz S.J., Ross S.E., Henson R.A., Perrin D.H.
    doi: 10.11138/mltj/2018.8.2.238
  14. Ligaments vs tendons in joint reconstruction: a review of histology and biomechanics
    Ge S.M., Alnaif N., Azzi A.J., Zadeh T.
    doi: 10.11138/mltj/2018.8.2.246
  15. The immediate effects of whole body vibration on cervical joint position sense in subjects with forward head posture
    Salami A., Roostayi M.M., Sadat Naimi S., Shadmehr A., Baghban A.A.
    doi: 10.11138/mltj/2018.8.2.255
  16. Muscular injuries after tendon rupture in the rotator cuff of animal models. Systematic review
    Terrón V.M., Muiños-López E., Granero-Moltó F., Esteban M.A., Prosper F., Pérez-Ruiz A., Pons-Villanueva J.
    doi: 10.11138/mltj/2018.8.2.261
  17. Complete midsubstance rectus femoris ruptures: a series of 27 athletes treated operatively
    Lempainen L., Kosola J., Niemi P., Orava S., Pruna R.
    doi: 10.11138/mltj/2018.8.2.276
  18. Internal obturator muscle indirect injury in groin pain syndrome: a systematic review of the literature
    Bisciotti G.N., Corsini A., Vuckvovic Z., Cena E., Bisciotti A., Belli A., Quaglia A., Volpi P.
    doi: 10.11138/mltj/2018.8.2.283
  19. Potential MRI findings associated with inguinal hernia and inguinal canal posterior wall weakness in athletes
    Bisciotti G.N., Auci A., Cena E., Corsini A., Bisciotti A., Eirale C., Parra F., Gassaghi G., Di Marzo F., Vuckvovic Z., Quaglia A., Volpi P.
    doi: 10.11138/mltj/2018.8.2.290
Last Viewed articles: la lista degli ultimi x visitati.
  1. Hyperglycemia induces osteogenic differentiation of bone marrow derived stem cells: an in vitro study
    Giai Via A., McCarthy M.B., Francke M., Oliva F., Mazzocca A.D., Maffulli N.
    doi: 10.11138/mltj/2018.8.1.001
credits