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Muscle, Ligaments and Tendons Journal

Hyperglycemia induces osteogenic differentiation of bone marrow derived stem cells: an in vitro study

Original Article, 1 - 7
doi: 10.11138/mltj/2018.8.1.001
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Abstract
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Introduction: The physiopathology of calcific tendinopathy (CT) is largely unknown. It could be the result of an active cell-mediated process.
Many endocrine and metabolic diseases may impair the homeostasis of the tendon. The present study investigated whether hyperglycemia may influence the differentiation of bone marrow
MSCs (bMSCs). The hypothesis is that high glucose levels may induce bone differentiation of MSCs.
Methods: Bone marrow (BM) was aspirated from the humeral head of three patients and concentrated. bMSCs were taken to the lab, counted, plated and grown to confluence. After 24 hrs cells were treated with MEM supplemented with low (5.0 mM), physiological (10 m M) and high (25 mM) glucose, (+) and (-) 10 m M insulin. Control cells were treated with MEM alone. Quantitative polymerase chain reaction (qPCR) was used to measure changes in gene expression levels specific for fibrocartilage in bMSCs.
Results: After 7 days, a significantly increased gene expression of collagen type I, type II, alkaline phosphatase and osteopontin was found in bMSCs supplemented with high glucose compared both to control group, and to low and physiological glucose groups with and without insulin (p<0.05). When insulin was added to high glucose culture, a significantly higher expression of aggrecan, alkaline phosphatase, type I and II collagen, and fibronectin was found compared to all the other groups (p<0.05).
Conclusion: When cultured in a high glucose medium, bMSCs express bone markers, and are able to differentiate toward an osteoblast lineage. CT may be caused by erroneous differentiation of MSCs in presence of high glucose serum levels.

Vol. 8 (No. 1) 2018 January/March

  1. Hyperglycemia induces osteogenic differentiation of bone marrow derived stem cells: an in vitro study
    Giai Via A., McCarthy M.B., Francke M., Oliva F., Mazzocca A.D., Maffulli N.
    doi: 10.11138/mltj/2018.8.1.001
  2. Identification of differentially expressed micro-RNA in rotator cuff tendinopathy
    Hall K.E., Sarkissian E.J., Sharpe O., Robinson W.H., Abrams G.D.
    doi: 10.11138/mltj/2018.8.1.008
  3. Are COL1A1 gene polymorphisms associated with anterior cruciate ligament tear in the Indian population? Results of a preliminary case-control study
    Prabhakar S., John R., Dhillon M.S., Anand A., Sharma K., Bmmidi S.
    doi: 10.11138/mltj/2018.8.1.015
  4. MRI evaluation of anterolateral ligament tears in knee injury with anterior cruciate ligament rupture
    Hooda A., Dhillon M.S., Prabhakar S., Prakash M., John R., Kanwat H.
    doi: 10.11138/mltj/2018.8.1.023
  5. Effect of post-activation potentiation induced by one, two or three half-squats on repeated sprint acceleration performance
    Rouissi M., Turki O., Bragazzi N.L., Owen A., Haddad M., Chamari K., Chtara M.
    doi: 10.11138/mltj/2018.8.1.028
  6. Acute effects of stretching duration on sprint performance of adolescent football players
    Iatridou G., Dionyssiotis Y., Papathanasiou J., Kapetanakis S., Galitsanos S.
    doi: 10.11138/mltj/2018.8.1.037
  7. The effect of deep shoulder infections on patient outcomes after arthroscopic rotator cuff repair: a retrospective comparative study
    Atesok K., MacDonald P., Leiter J., McRae S., Singh M., Stranges G., Old J.
    doi: 10.11138/mltj/2018.8.1.043
  8. Arthroscopic versus open Latarjet in the treatment of recurrent anterior shoulder instability: a prospective study
    Savo A., Lanzetti R.M., Topa D., Campagna V., Pulcinelli F., Spoliti M.
    doi: 10.11138/mltj/2018.8.1.051
  9. Musculotendinous equinus deformity correction: gastrocnemius or gastrosoleus release
    De Luna V., Oliva F., Lo Bue G., Tudisco C., Farsetti P.
    doi: 10.11138/mltj/2018.8.1.058
  10. Sex-related differences in plantarflexor function during repeated stretch-shortening cycle loading
    Furlong L.-A. M, Harrison A.J.
    doi: 10.11138/mltj/2018.8.1.076
  11. Patellar and quadriceps tendon abnormalities in obese patients with knee osteoarthritis: a preliminary ultrasonographic study
    Benedetti M.G., Frizziero A., Affinito D., Cavazzuti L., Bonfiglioli Stagni S., Galletti S., Catani F., Maffulli N.
    doi: 10.11138/mltj/2018.8.1.085
  12. A novel integrative approach to improve the quality of life by reducing pain and kinesiophobia in patients undergoing TKA: the IARA Model
    Padovan A.M., Oprandi G., Padulo J., Bruno C., Isoardi M., Gulotta F., Kuvačić G., De Giorgio A.
    doi: 10.11138/mltj/2018.8.1.093
  13. Effectiveness of yoga combined with back school program on anxiety, kinesiophobia and pain in people with non-specific chronic low back pain: a prospective randomized trial
    De Giorgio A., Padulo J., Kuvačić G.
    doi: 10.11138/mltj/2018.8.1.104
  14. Lower limb strength, but not sensorial integration, explains the age-associated postural control impairment
    Andrade H.B., Costa S.M., Pirôpo U.S., Schettino L., Casotti C.A., Pereira R.
    doi: 10.11138/mltj/2018.8.1.113
  15. Minimally invasive release of plantar fascia. A clinical retrospective study
    Poutoglidou F., Papadopoulos A., Kalinderis A., Koukos A.
    doi: 10.11138/mltj/2018.8.1.118
  16. Prognosis of conservative treatment in individuals with temporomandibular disorders and tinnitus: a systematic review
    Feitoza C.C., de Lemos Menezes P.
    doi: 10.11138/mltj/2018.8.1.123
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  1. Hyperglycemia induces osteogenic differentiation of bone marrow derived stem cells: an in vitro study
    Giai Via A., McCarthy M.B., Francke M., Oliva F., Mazzocca A.D., Maffulli N.
    doi: 10.11138/mltj/2018.8.1.001
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