Cic edizioni internazionali
Muscle, Ligaments and Tendons Journal

Identification of differentially expressed micro-RNA in rotator cuff tendinopathy

Original Article, 8 - 14
doi: 10.11138/mltj/2018.8.1.008
Tag this article
Abstract
Full text PDF
Introduction. The pathomolecular events behind the development of tendinopathy remain unknown.
Methods. Paired biopsies from human shoulder supraspinatus (tendinopathy) and subscapularis (control) tendons were obtained from patients undergoing shoulder arthroscopy, with tendinopathy
confirmed using light microscopy evaluation.
Differentially expressed micro-RNA were identified using RNA sequencing analysis, with target genes for the differentially expressed micro-RNA predicted using miRbase and Targetscan. The micro-RNA were secondarily screened to identify those with predicted binding to genes known be involved in tendinopathy – collagen 1A1/1A2/3A1, BMP-2/7, and IL-6 – with additional cross referencing of gene/micro-RNA predicted binding performed with NetworkAnalyst.
Results. Supraspinatus samples demonstrated significantly greater histologic characteristics of tendinopathy versus subscapularis samples. A total of 21 micro-RNA were differentially expressed between normal and diseased tendon, with six (let-7g, miR-7a, miR-22, miR-26a/b, and miR-29a) having predicted binding to genes known to be involved in tendinopathy.
Conclusion. The identiied micro-RNAs may be involved in the development of tendon disease and represent potential therapeutic targets. Level of evidence: Basic science investigation.

Vol. 8 (No. 4) 2018 October/December

  1. Chondrocalcinosis: a morphofunctional study of crystal deposition in mechanically stressed shoulder soft tissues
    Burini D., Salucci S., Fardetti F., Beccarini A., Calvisi V., Gobbi P., Falcieri E., Curzi D.
    doi: 10.11138/mltj/2018.8.4.465
  2. Functional hyper-extension deficit of the knee and patellar dimple sign in patellofemoral pain syndrome. A preliminary study
    Kaya D., Aksahin E., Doral M.N.
    doi: 10.11138/mltj/2018.8.4.473
  3. The triple F knot: a sliding-locking arthroscopic knot
    Spezia F., Franceschetti E., Franceschi F., Maffulli N., Oliva F.
    doi: 10.11138/mltj/2018.8.4.477
  4. MRI scans do not accurately predict hamstring graft diameter for ACL reconstruction
    Ricci P., Cullen C., Imalingat H., Sergeant J.C., Jain N.
    doi: 10.11138/mltj/2018.8.4.482
  5. Treating Haglund's Deformity with percutaneous Achilles tendon decompression: a case series
    Sergio F., Catani O., Fusini F., Langella F., Cautiero G., Ponzo I., Zanchini F.
    doi: 10.11138/mltj/2018.8.4.488
  6. Structured training volume in early adolescence: a risk factor for femoroacetabular impingement?
    Mullins K., Hanlon M., Carton P.
    doi: 10.11138/mltj/2018.8.4.495
  7. Relationship between anthropometric characteristics and playing position in youth soccer
    Notarnicola A., Maccagnano G., Chierico A., Tafuri S., Moretti B.
    doi: 10.11138/mltj/2018.8.4.502
  8. Posture and scapular dyskinesis in young bowed string instrumental musicians
    Frizziero A., Gasparre G., Corvo S., Gamberini J., Finotti P., Masiero S., Maffulli N.
    doi: 10.11138/mltj/2018.8.4.507
  9. Shoulder external/internal rotation peak torques ratio side-asymmetry, mean work and power ratios balance worsening due to different fatigue resistance of the rotator muscles in male handball players
    Pontaga I.
    doi: 10.11138/mltj/2018.8.4.513
  10. Does TRX training induce similar effects to crossfit? Study on the variation of body fat mass, endurance and explosive force
    Notarnicola A., Salatino G., Napoletano P., Monno A., Moretti B., Tafuri S.
    doi: 10.11138/mltj/2018.8.4.520
  11. The therapeutic effects of manipulation technique on sacroiliac joint dysfunction in young women
    Ashraf V., Shadmehr A., Moghadam B.A., Olyaei G., Reza Hadian M., Khazaeipour Z.
    doi: 10.11138/mltj/2018.8.4.526
  12. Changes of joint position sense in responses to upper trapezius muscle fatigue in subclinical myofascial pain syndrome participants versus healthy control
    Roya M., Gholam Reza O., Azadeh S., Reza H.M., Saeed T.M.
    doi: 10.11138/mltj/2018.8.4.534
  13. Extruded collagen fibres and biphasic collagen sponge for rotator cuff tendon repair application: an ovine in vitro study
    Zafar A., Rushton N.
    doi: 10.11138/mltj/2018.8.4.544
  14. The comparison of dynamic postural control and muscle activity in time domain in athletes with and without chronic ankle instability
    Fereydounnia S., Shadmehr A., Talebian Moghadam S., Olyaei G., Jalaie S., Shiravi Z., Salemi S.
    doi: 10.11138/mltj/2018.8.4.552
Last Viewed articles: la lista degli ultimi x visitati.
  1. Identification of differentially expressed micro-RNA in rotator cuff tendinopathy
    Hall K.E., Sarkissian E.J., Sharpe O., Robinson W.H., Abrams G.D.
    doi: 10.11138/mltj/2018.8.1.008
credits