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Muscle, Ligaments and Tendons Journal

Identification of differentially expressed micro-RNA in rotator cuff tendinopathy

Original Article, 8 - 14
doi: 10.11138/mltj/2018.8.1.008
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Abstract
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Introduction. The pathomolecular events behind the development of tendinopathy remain unknown.
Methods. Paired biopsies from human shoulder supraspinatus (tendinopathy) and subscapularis (control) tendons were obtained from patients undergoing shoulder arthroscopy, with tendinopathy
confirmed using light microscopy evaluation.
Differentially expressed micro-RNA were identified using RNA sequencing analysis, with target genes for the differentially expressed micro-RNA predicted using miRbase and Targetscan. The micro-RNA were secondarily screened to identify those with predicted binding to genes known be involved in tendinopathy – collagen 1A1/1A2/3A1, BMP-2/7, and IL-6 – with additional cross referencing of gene/micro-RNA predicted binding performed with NetworkAnalyst.
Results. Supraspinatus samples demonstrated significantly greater histologic characteristics of tendinopathy versus subscapularis samples. A total of 21 micro-RNA were differentially expressed between normal and diseased tendon, with six (let-7g, miR-7a, miR-22, miR-26a/b, and miR-29a) having predicted binding to genes known to be involved in tendinopathy.
Conclusion. The identiied micro-RNAs may be involved in the development of tendon disease and represent potential therapeutic targets. Level of evidence: Basic science investigation.

Vol. 8 (No. 2) 2018 April/June

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