Cic edizioni internazionali
Muscle, Ligaments and Tendons Journal

Lactoferrin and parathyroid hormone are not harmful to primary tenocytes in vitro, but PDGF may be

Original Article, 215 - 222
doi: 10.11138/mltj/2017.7.2.215
Tag this article
Abstract
Enhanced HTML Full text PDF
Introduction: Recently, bone-active factors such as parathyroid hormone and lactoferrin, have been used in pre-clinical models to promote tendon healing. How-ever, there is limited understanding of how these boneactive factors may affect the cells of the ten-don themselves. Here, we present an in vitro study assessing the effects of parathyroid hor-mone and lactoferrin on primary tendon cells (tenocytes), and compare their responses to the tenogenic factors, PDGF, IGF-1 and TGF-β.
Materials and Methods: Tenocyte proliferation and collagen production were assessed by alamarBlue ® and Sirius red as-says, respectively. To assess tenocyte trans-differentiation, changes in the expression of genes important in tenocyte, chondrocyte and osteoblast biology were determined using real-time PCR.
Results: Parathyroid hormone and lactoferrin had no effect on tenocyte growth or collagen production, with minimal changes in gene expression and no detrimental effects observed to suggest trans-differentiation away from tendon cell behaviour.
Tenogenic factors PDGF, IGF-1 and TGF all increasetenocyte collagen production, however, the gene expression data suggests that PDGF promotes severe de-differentiation of the tenocytes.
Discussion: Our findings suggest that using parathyroid hormone or lactoferrin as a singular factor to promote tendon healing may not be of benefit, but for use in tendon-bone healing there would be no detrimental effect on the tendon itself.

Vol. 8 (No. 2) 2018 April/June

  1. Percutaneous trigger finger release with an MS64 scalpel
    Mifsut-Miedes D., Valverde Navarro A.A., Rivelles J.R.
    doi: 10.11138/mltj/2018.8.2.135
  2. The effect of choice reaction time task on pre-landing muscle timing in athletes with and without chronic ankle instability
    Fereydounnia S., Shadmehr A., Talebian Moghadam S., Olyaei G., Jalaie S.
    doi: 10.11138/mltj/2018.8.2.142
  3. Cigarette smoking on the functional recovery of patients with rotator cuff tear submitted to physical therapy
    Cardoso Leffa T., Galvão Novelli J., Biff dos Santos G., Maciel Bello G., da Silva Santos M., Martins Silveira M., Leindecker R.C., Boff Daitx R., Baptista Dohnert M.
    doi: 10.11138/mltj/2018.8.2.150
  4. The undefined anatomical variations of the deltoid ligament bundles: a cadaveric study
    Zamperetti M., Guelfi M., Biz C., Pantalone A., Salini V., Oliva X.M., Ruggieri P., Mirapeix R.M.
    doi: 10.11138/mltj/2018.8.2.163
  5. The reproducibility of manual and fixed tensioning in anterior cruciate ligament reconstruction
    Stevens R., Chandrasenan J., Ali F., Haslam P., Nicolaou N.
    doi: 10.11138/mltj/2018.8.2.172
  6. Arthroscopic management of anterior inferior iliac spine impingement: a systematic review
    Fioruzzi A., Jannelli E., Ivone A., Perelli S., Fontana A., Giai Via A.
    doi: 10.11138/mltj/2018.8.2.178
  7. Low revision rate and excellent subjective outcome of primary ACL repair with a minimum follow-up of 5 years
    Nau T., Teuschl A., Ebner A., Jung I., Schenk C.
    doi: 10.11138/mltj/2018.8.2.185
  8. Box-loop technique in the management of complex elbow instability: a prospective controlled trial
    Vicenti G., Solarino G., Carrozzo M., Bizzoca D., Marinelli A., Rotini R., Moretti B.
    doi: 10.11138/mltj/2018.8.2.191
  9. Simvastatin does not adversely affect Achilles tendon properties in a diet induced hypercholesterolemia rat model
    Choi D.S., Shetye S.S., Tucker J.J., Huegel J., Soslowsky L.J.
    doi: 10.11138/mltj/2018.8.2.199
  10. Quality assessment of muscle injury classification in sports: a systematic literature review
    SantAnna J.P.C., de Almeida A.M., Pedrinelli A., Hernandez A.J., Fernandes T.L.
    doi: 10.11138/mltj/2018.8.2.206
  11. Laser therapy penetration depth: a near-infrared study on a horse tendon model
    Monici M., Gnerucci A., Falconi T., Bani D., Cialdai F., Fusi F., Romano G.
    doi: 10.11138/mltj/2018.8.2.222
  12. Quantitative elastography of Achilles tendon using Shear Wave Elastography (SWE): correlation with zonal anatomy
    Petitpierre F., Perez J.T., Bise S., Fournier C., Hauger O., Dallaudière B.
    doi: 10.11138/mltj/2018.8.2.229
  13. Regional differences in anterior cruciate ligament imaging biomarkers: T2 and T2* values
    Schmitz R.J., Wang H-M., Kraft R.A., Shultz S.J., Ross S.E., Henson R.A., Perrin D.H.
    doi: 10.11138/mltj/2018.8.2.238
  14. Ligaments vs tendons in joint reconstruction: a review of histology and biomechanics
    Ge S.M., Alnaif N., Azzi A.J., Zadeh T.
    doi: 10.11138/mltj/2018.8.2.246
  15. The immediate effects of whole body vibration on cervical joint position sense in subjects with forward head posture
    Salami A., Roostayi M.M., Sadat Naimi S., Shadmehr A., Baghban A.A.
    doi: 10.11138/mltj/2018.8.2.255
  16. Muscular injuries after tendon rupture in the rotator cuff of animal models. Systematic review
    Terrón V.M., Muiños-López E., Granero-Moltó F., Esteban M.A., Prosper F., Pérez-Ruiz A., Pons-Villanueva J.
    doi: 10.11138/mltj/2018.8.2.261
  17. Complete midsubstance rectus femoris ruptures: a series of 27 athletes treated operatively
    Lempainen L., Kosola J., Niemi P., Orava S., Pruna R.
    doi: 10.11138/mltj/2018.8.2.276
  18. Internal obturator muscle indirect injury in groin pain syndrome: a systematic review of the literature
    Bisciotti G.N., Corsini A., Vuckvovic Z., Cena E., Bisciotti A., Belli A., Quaglia A., Volpi P.
    doi: 10.11138/mltj/2018.8.2.283
  19. Potential MRI findings associated with inguinal hernia and inguinal canal posterior wall weakness in athletes
    Bisciotti G.N., Auci A., Cena E., Corsini A., Bisciotti A., Eirale C., Parra F., Gassaghi G., Di Marzo F., Vuckvovic Z., Quaglia A., Volpi P.
    doi: 10.11138/mltj/2018.8.2.290
Last Viewed articles: la lista degli ultimi x visitati.
  1. Lactoferrin and parathyroid hormone are not harmful to primary tenocytes in vitro, but PDGF may be
    Musson D.S., Tay M.L., Chhana A., Pool B., Coleman B, Naot D., Cornish J.
    doi: 10.11138/mltj/2017.7.2.215
credits