Cic edizioni internazionali
Muscle, Ligaments and Tendons Journal

Lactoferrin and parathyroid hormone are not harmful to primary tenocytes in vitro, but PDGF may be

Original Article, 215 - 222
doi: 10.11138/mltj/2017.7.2.215
Tag this article
Abstract
Enhanced HTML Full text PDF
Introduction: Recently, bone-active factors such as parathyroid hormone and lactoferrin, have been used in pre-clinical models to promote tendon healing. How-ever, there is limited understanding of how these boneactive factors may affect the cells of the ten-don themselves. Here, we present an in vitro study assessing the effects of parathyroid hor-mone and lactoferrin on primary tendon cells (tenocytes), and compare their responses to the tenogenic factors, PDGF, IGF-1 and TGF-β.
Materials and Methods: Tenocyte proliferation and collagen production were assessed by alamarBlue ® and Sirius red as-says, respectively. To assess tenocyte trans-differentiation, changes in the expression of genes important in tenocyte, chondrocyte and osteoblast biology were determined using real-time PCR.
Results: Parathyroid hormone and lactoferrin had no effect on tenocyte growth or collagen production, with minimal changes in gene expression and no detrimental effects observed to suggest trans-differentiation away from tendon cell behaviour.
Tenogenic factors PDGF, IGF-1 and TGF all increasetenocyte collagen production, however, the gene expression data suggests that PDGF promotes severe de-differentiation of the tenocytes.
Discussion: Our findings suggest that using parathyroid hormone or lactoferrin as a singular factor to promote tendon healing may not be of benefit, but for use in tendon-bone healing there would be no detrimental effect on the tendon itself.

Vol. 8 (No. 4) 2018 October/December

  1. Chondrocalcinosis: a morphofunctional study of crystal deposition in mechanically stressed shoulder soft tissues
    Burini D., Salucci S., Fardetti F., Beccarini A., Calvisi V., Gobbi P., Falcieri E., Curzi D.
    doi: 10.11138/mltj/2018.8.4.465
  2. Functional hyper-extension deficit of the knee and patellar dimple sign in patellofemoral pain syndrome. A preliminary study
    Kaya D., Aksahin E., Doral M.N.
    doi: 10.11138/mltj/2018.8.4.473
  3. The triple F knot: a sliding-locking arthroscopic knot
    Spezia F., Franceschetti E., Franceschi F., Maffulli N., Oliva F.
    doi: 10.11138/mltj/2018.8.4.477
  4. MRI scans do not accurately predict hamstring graft diameter for ACL reconstruction
    Ricci P., Cullen C., Imalingat H., Sergeant J.C., Jain N.
    doi: 10.11138/mltj/2018.8.4.482
  5. Treating Haglund's Deformity with percutaneous Achilles tendon decompression: a case series
    Sergio F., Catani O., Fusini F., Langella F., Cautiero G., Ponzo I., Zanchini F.
    doi: 10.11138/mltj/2018.8.4.488
  6. Structured training volume in early adolescence: a risk factor for femoroacetabular impingement?
    Mullins K., Hanlon M., Carton P.
    doi: 10.11138/mltj/2018.8.4.495
  7. Relationship between anthropometric characteristics and playing position in youth soccer
    Notarnicola A., Maccagnano G., Chierico A., Tafuri S., Moretti B.
    doi: 10.11138/mltj/2018.8.4.502
  8. Posture and scapular dyskinesis in young bowed string instrumental musicians
    Frizziero A., Gasparre G., Corvo S., Gamberini J., Finotti P., Masiero S., Maffulli N.
    doi: 10.11138/mltj/2018.8.4.507
  9. Shoulder external/internal rotation peak torques ratio side-asymmetry, mean work and power ratios balance worsening due to different fatigue resistance of the rotator muscles in male handball players
    Pontaga I.
    doi: 10.11138/mltj/2018.8.4.513
  10. Does TRX training induce similar effects to crossfit? Study on the variation of body fat mass, endurance and explosive force
    Notarnicola A., Salatino G., Napoletano P., Monno A., Moretti B., Tafuri S.
    doi: 10.11138/mltj/2018.8.4.520
  11. The therapeutic effects of manipulation technique on sacroiliac joint dysfunction in young women
    Ashraf V., Shadmehr A., Moghadam B.A., Olyaei G., Reza Hadian M., Khazaeipour Z.
    doi: 10.11138/mltj/2018.8.4.526
  12. Changes of joint position sense in responses to upper trapezius muscle fatigue in subclinical myofascial pain syndrome participants versus healthy control
    Roya M., Gholam Reza O., Azadeh S., Reza H.M., Saeed T.M.
    doi: 10.11138/mltj/2018.8.4.534
  13. Extruded collagen fibres and biphasic collagen sponge for rotator cuff tendon repair application: an ovine in vitro study
    Zafar A., Rushton N.
    doi: 10.11138/mltj/2018.8.4.544
  14. The comparison of dynamic postural control and muscle activity in time domain in athletes with and without chronic ankle instability
    Fereydounnia S., Shadmehr A., Talebian Moghadam S., Olyaei G., Jalaie S., Shiravi Z., Salemi S.
    doi: 10.11138/mltj/2018.8.4.552
Last Viewed articles: la lista degli ultimi x visitati.
  1. Lactoferrin and parathyroid hormone are not harmful to primary tenocytes in vitro, but PDGF may be
    Musson D.S., Tay M.L., Chhana A., Pool B., Coleman B, Naot D., Cornish J.
    doi: 10.11138/mltj/2017.7.2.215
credits