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The molecular systemic and local effects of intratendinous injection of Platelet Rich Plasma in
tendinosis: preliminary results on a rat model with ELISA method

Dallaudiere B., Louedec L., Lenet M.P.J., Pesquer L., Blaise E., Perozziello A., Michel J.B., Moinard M., Meyer P., Serfaty J.M.
Original Article, 99 - 105
doi: 10.11138/mltj/2015.5.2.099
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Abstract
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Purpose: the aim of our study was thus to quantify the effect of Platelet Rich Plasma (PRP) injection on systemic and local growth factors and to identify molecular markers in a rat model of patellar and Achilles tendinosis treated with PRP.
Material and method: twenty two rats were used for the study. Two healthy rats were used as control (T-). We induced tendinosis (T+) in 20 rats (80 tendons by injecting under ultrasonography (US) guidance Collagenase 1® (day 0 = D0, patellar=40 and Achilles=40).
At D3, these 20 rats with tendinosis were separated in treatment by either PRP (PRPT+, n=28), physiological serum (PST+, n=28, control) USguided intratendinous injection, or without no PRP or PS (T+, n=24, control of natural evolution of tendinopathy. Follow-up at D7, D13, D18 and D25 using serum sample and local tendon removal with ELISA technics and comparison between the 3 groups were performed.
Results: during biological follow up, comparison of all serum samples of PRPT+, PST+ and T+ groups showed no significant modification of their biological markers at D7, D13, D18 and D25 (p>0.22). Comparison of immunological sample tendon markers of PRPT+, PST+ and T+ groups also showed no significant modification of markers at D7, D13, D18 and D25 (p>0.16) considering each biological marker and also all subgroups confounded.
Conclusion: our study strongly suggests that a single intratendinous US-guided injection of PRP in Achilles and patellar T+ doesn’t increase biological markers such as growth factors compared to a control group in mid-term and long-term follow-up.
KEY WORDS: tendinosis, rat, platelet, PRP, ELISA.

Vol. 5 (No. 2) 2015 April/June

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  1. The molecular systemic and local effects of intratendinous injection of Platelet Rich Plasma in
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    Dallaudiere B., Louedec L., Lenet M.P.J., Pesquer L., Blaise E., Perozziello A., Michel J.B., Moinard M., Meyer P., Serfaty J.M.
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